prm1 rabbit polyclonal antibody  (Sangon Biotech)

Journal: Frontiers in Cell and Developmental Biology

Article Title: Role of Phosphorylated Gonadotropin-Regulated Testicular RNA Helicase (GRTH/DDX25) in the Regulation of Germ Cell Specific mRNAs in Chromatoid Bodies During Spermatogenesis

doi: 10.3389/fcell.2020.580019

Figure Lengend Snippet: Binding of GRTH to specific germ cell mRNAs in chromatoid bodies (CBs) isolated from wild-type (WT) and knock-in (KI) mice. Relative binding of Tnp1/2 , Prm1/2 , Grth , and Tssk6 mRNA to GRTH protein in CB of germ cells in the testes from WT and GRTH-KI mice. All statistical analyses were performed using Student’s t -test (* P < 0.05), and data represent mean ± SEM of three independent experiments in triplicates.

Article Snippet: The membrane was blocked with 5% skimmed milk powder in tris-buffered saline and then incubated with either of the following primary antibodies: affinity-purified anti-GRTH rabbit polyclonal antibody (1:500 dilution), anti-MVH/DDX4 polyclonal (1:500; cst#8761; Cell Signaling), mouse Argonaute/PIWI family RNA binding protein (MIWI) monoclonal antibody (CB control; 1:500; cst#6915; Cell Signaling), brain and muscle ARNT-like 1 (BMAL1) rabbit monoclonal antibody (1:500 dilution; cst#14020; Cell Signaling), circadian locomotor output cycles protein kaput (CLOCK) rabbit monoclonal antibody (1:500 dilution; cst#5157; Cell Signaling), Ybx3 mouse monoclonal antibody (1:500 dilution; LS-C105064; LS Bio), PRM1 rabbit polyclonal antibody (1:500 dilution; HPA055150; Millipore Sigma, St. Louis, MO, United States), and PRM2 rabbit monoclonal antibody (1:500 dilution; Sc-30172; Santa Cruz Biotechnology, Dallas, Texas).

Techniques: Binding Assay, Isolation, Knock-In